Shi, QiuyingIbrahim, AshleyHerbert, KristiCarvin, MarciaRandolph, MelissaPost, Kristin M.Curless, KendraChen, ShaoxiongCramer, Harvey M.Cheng, LiangWu, Howard H.2015-12-222015-12-222015-04Shi, Q., Ibrahim, A., Herbert, K., Carvin, M., Randolph, M., Post, K. M., … Wu, H. H. (2015). Detection of BRAF Mutations on Direct Smears of Thyroid Fine-Needle Aspirates Through Cell Transfer Technique. American Journal of Clinical Pathology, 143(4), 500–504. http://doi.org/10.1309/AJCP5BG0KUEOJCVShttps://hdl.handle.net/1805/7793Objectives: To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. Methods: Polymerase chain reaction (PCR)–based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases. Results: BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues. Conclusions: PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT.en-USPublisher Policythyroidcytologycell transferArticle