Menniti, MirandaIuliano, RodolfoFöller, MichaelSopjani, MentorAlesutan, IoanaMariggiò, StefaniaNofziger, CharityPerri, Angela M.Amato, RosarioBlazer-Yost, BonnieCorda, DanielaLang, FlorianPerrotti, Nicola2018-08-152018-08-152010Miranda Menniti, Rodolfo Iuliano, Michael Föller, Mentor Sopjani, Ioana Alesutan, Stefania Mariggiò, Charity Nofziger, Angela M. Perri, Rosario Amato, Bonnie Blazer-Yost, Daniela Corda, Florian Lang, and Nicola Perrotti. 60kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC. Cellular Physiology and Biochemistry. (2010).https://hdl.handle.net/1805/17146The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of ENaC-mediated sodium transport and is involved in the transduction of growth-factor-dependent cell survival and proliferation. The identification of molecular partners for Sgk1 is crucial for the understanding of its mechanisms of action. We performed a yeast two-hybrid screening based on a human kidney cDNA library to identify molecular partners of Sgk1. As a result the screening revealed a specific interaction between Sgk1 and a 60 kDa Lysophospholipase (LysoLP). LysoLP is a poorly characterized enzyme that, based on sequence analysis, might possess lysophospholipase and asparaginase activities. We demonstrate that LysoLP has indeed a lysophospholipase activity and affects metabolic functions related to cell proliferation and regulation of membrane channels. Moreover we demonstrate in the Xenopus oocyte expression system that LysoLP downregulates basal and Sgk1-dependent ENaC activity. In conclusion LysoLP may represent a new player in the regulation of ENaC and Sgk1-dependent signaling.Epithelial Sodium ChannelsImmediate-Early ProteinsLysophospholipaseProtein-Serine-Threonine KinasesArticle