Commodore, SarahSharma, ShikhaEkpruke, Carolyn DamilolaPepin, RobertHansen, Angela M.Rousselle, DustinBabayev, MaksatNdeke, Jonas M.Alford, RachelParker, ErikDickinson, StephanieSharma, SunitaSilveyra, Patricia2024-07-312024-07-312023Commodore S, Sharma S, Ekpruke CD, et al. Thirdhand vaping exposures are associated with pulmonary and systemic inflammation in a mouse model. J Environ Expo Assess. 2023;2(4):22. doi:10.20517/jeea.2023.27https://hdl.handle.net/1805/42498Thirdhand smoke (THS) is the accumulation of secondhand smoke on surfaces that ages with time. THS exposure is a potential health threat to children, partners of smokers, and workers in environments with current or past smoking, and needs further investigation. In this study, we hypothesized that thirdhand Electronic Nicotine Delivery Systems (ENDS) exposures elicit lung and systemic inflammation due to resuspended particulate matter (PM) and inorganic compounds that remain after active vaping has ceased. To test our hypothesis, we exposed C57BL/6J mice to cotton towels contaminated with ENDS aerosols from unflavored vape fluid (6 mg nicotine in 50/50 propylene glycol/vegetable glycerin) for 1h/day, five days/week, for three weeks. We assessed protein levels in serum and bronchoalveolar lavage fluid (BALF) using a multiplex protein assay. The mean ± sd for PM10 and PM2.5 measurements in exposed mouse cages were 8.3 ± 14.0 and 4.6 ± 7.5 μg/m3, compared to 6.1 ± 11.2 and 3.7 ± 6.6 μg/m3 in control cages respectively. Two compounds, 4-methyl-1, 2-dioxolane and 4-methyl-cyclohexanol, were detected in vape fluid and on ENDS-contaminated towels, but not on control towels. Mice exposed to ENDS-contaminated towels had lower levels of serum Il-7 (P = 0.022, n = 7), and higher levels of Il-13 in the BALF (P = 0.006, n = 7) than those exposed to control towels (n = 6). After adjusting for sex and age, Il-7 and Il-13 levels were still associated with thirdhand vaping exposure (P = 0.010 and P = 0.017, respectively). This study provides further evidence that thirdhand ENDS aerosols can contaminate surfaces, and subsequently influence lung and systemic health upon exposure.en-USPublisher PolicyE-cigarettesBlood cytokinesLung cytokinesMouse modelParticulate matterOrganic chemicalsThirdhand smokeVapingArticle