Wang, JihuaYang, YuedongCao, ZanxiaLi, ZhixiuZhao, HuiyingZhou, Yaoqi2016-03-032016-03-032013-12-03Wang, J., Yang, Y., Cao, Z., Li, Z., Zhao, H., & Zhou, Y. (2013). The Role of Semidisorder in Temperature Adaptation of Bacterial FlgM Proteins. Biophysical Journal, 105(11), 2598–2605. http://doi.org/10.1016/j.bpj.2013.10.026https://hdl.handle.net/1805/8681Probabilities of disorder for FlgM proteins of 39 species whose optimal growth temperature ranges from 273 K (0°C) to 368 K (95°C) were predicted by a newly developed method called Sequence-based Prediction with Integrated NEural networks for Disorder (SPINE-D). We showed that the temperature-dependent behavior of FlgM proteins could be separated into two subgroups according to their sequence lengths. Only shorter sequences evolved to adapt to high temperatures (>318 K or 45°C). Their ability to adapt to high temperatures was achieved through a transition from a fully disordered state with little secondary structure to a semidisordered state with high predicted helical probability at the N-terminal region. The predicted results are consistent with available experimental data. An analysis of all orthologous protein families in 39 species suggests that such a transition from a fully disordered state to semidisordered and/or ordered states is one of the strategies employed by nature for adaptation to high temperatures.en-USPublisher PolicyAdaptation, PhysiologicalAlgorithmsAmino Acid SequenceBacteriaBacterial ProteinsHot TemperatureMolecular Dynamics SimulationMolecular Sequence DataProtein ConformationSpecies SpecificityArticle