Matos, Adaias OliveiraBarão, Valentim Adelino RicardoGregory, Richard Lee2024-09-262024-09-262023Matos AO, Barão VAR, Gregory RL. Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms. Braz J Oral Sci. 2023;22:e231137. doi:10.20396/bjos.v22i00.8671137https://hdl.handle.net/1805/43631Aim: The purpose of this in vitro study was to analyze the influence of nicotine on the extracellular polysaccharides in Fusobacterium nucleatum biofilm. Methods: F. nucleatum (ATCC 10953) biofilms supplemented with different concentrations of nicotine (0, 0.5, 1, 2, 4, and 8 mg/mL) were grown in two different BHI broth conditions [no sucrose and 1% sucrose]. Extracellular polysaccharides assay, pH measurements, and a spectrophotometric assay were performed. Data were submitted for ANOVA and Tukey honestly significant difference analyses (HSD) tests (α =.05). Results: Extracellular polysaccharides synthesis was influenced by an interaction between nicotine concentrations and growth medium solution containing sucrose (P<.05). The pH values declined in the sucrose-exposed biofilm were greater than in the group exposed only to nicotine (P<.05). The biofilm exposed to sucrose and nicotine had a higher total biofilm growth (P<.05) than the nicotine-treated biofilm without sucrose. Conclusions: Regardless of sucrose exposure, biofilms exposed to different nicotine concentrations influenced the amount of extracellular polysaccharides.en-USAttribution 4.0 InternationalBacterialBiofilmsNicotinePolysaccharidesArticle