Zandona, F.Soini, H. A.Novotny, M. V.Santiago, E.Eckert, G. J.Preisser, J. S.Benecha, H. K.Arthur, R. A.Zero, D. T.2017-09-212017-09-212015-03Zandona, F., Soini, H., Novotny, M., Santiago, E., Eckert, G., Preisser, J., … Zero, D. (2015). A Potential Biofilm Metabolite Signature for Caries Activity - A Pilot Clinical Study. Metabolomics : Open Access, 5(1), 140. http://doi.org/10.4172/2153-0769.10001402153-0769https://hdl.handle.net/1805/14154BACKGROUND: This study's aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. METHODS: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. RESULTS: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. CONCLUSIONS: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.en-USAttribution 3.0 United StatesBiomarkerCaries-activityDental-cariesGas chromatography-mass spectrometryMetabolitesArticle