Gourapura, Renukaradhya J.Khan, Masood A.Gallo, Richard M.Shaji, DanielLiu, JianyunBrutkiewicz, Randy R.2025-05-012025-05-012013-08-29Gourapura RJ, Khan MA, Gallo RM, Shaji D, Liu J, Brutkiewicz RR. Forming a complex with MHC class I molecules interferes with mouse CD1d functional expression. PLoS One. 2013;8(8):e72867. Published 2013 Aug 29. doi:10.1371/journal.pone.0072867https://hdl.handle.net/1805/47592CD1d molecules are structurally similar to MHC class I, but present lipid antigens as opposed to peptides. Here, we show that MHC class I molecules physically associate with (and regulate the functional expression of) mouse CD1d on the surface of cells. Low pH (3.0) acid stripping of MHC class I molecules resulted in increased surface expression of murine CD1d on antigen presenting cells as well as augmented CD1d-mediated antigen presentation to NKT cells. Consistent with the above results, TAP1-/- mice were found to have a higher percentage of type I NKT cells as compared to wild type mice. Moreover, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD1d compared to wild type mice. Together, these results suggest that MHC class I molecules can regulate NKT cell function, in part, by masking CD1d.en-USAttribution 4.0 InternationalCell membraneImmunophenotypingDendritic cellsLymphocyte activationArticle