Silberstein, LevGoncalves, Kevin A.Kharchenko, Peter V.Turcotte, RaphaelKfoury, YoumnaMercier, FrancoisBaryawno, NinibSevere, NicolasBachand, JacquelineSpencer, Joel A.Papazian, AniLee, DongjunChitteti, Brahmananda ReddySrour, Edward F.Hoggatt, JonathanTate, TiffanyLo Celso, CristinaOno, NoriakiNutt, StephenHeino, JyrkiSipilä, KalleShioda, ToshihiroOsawa, MasatakeLin, Charles P.Hu, Guo-FuScadden, David T.2020-02-062020-02-062016-10-06Silberstein, L., Goncalves, K. A., Kharchenko, P. V., Turcotte, R., Kfoury, Y., Mercier, F., … Scadden, D. T. (2016). Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators. Cell stem cell, 19(4), 530–543. doi:10.1016/j.stem.2016.07.004https://hdl.handle.net/1805/22005Physiological stem cell function is regulated by secreted factors produced by niche cells. In this study, we describe an unbiased approach based on differential single-cell gene expression analysis of mesenchymal osteolineage cells close to and further removed from hematopoietic stem/progenitor cells to identify candidate niche factors. Mesenchymal cells displayed distinct molecular profiles based on their relative location. Amongst the genes which were preferentially expressed in proximal cells, we functionally examined three secreted or cell surface molecules not previously connected to HSPC biology: the secreted RNase Angiogenin, the cytokine IL18 and the adhesion molecule Embigin and discovered that all of these factors are HSPC quiescence regulators. Our proximity-based differential single cell approach therefore reveals molecular heterogeneity within niche cells and can be used to identify novel extrinsic stem/progenitor cell regulators. Similar approaches could also be applied to other stem cell/niche pairs to advance understanding of microenvironmental regulation of stem cell function.en-USPublisher PolicyStem cell nicheSingle cell analysisHematopoiesisArticle