Wudiri, George A.Pritchard, Suzanne M.Li, HongLiu, JinAguilar, Hector C.Gilk, Stacey D.Nicola, Anthony V.2016-03-222016-03-222014-12Wudiri, G. A., Pritchard, S. M., Li, H., Liu, J., Aguilar, H. C., Gilk, S. D., & Nicola, A. V. (2014). Molecular Requirement for Sterols in Herpes Simplex Virus Entry and Infectivity. Journal of Virology, 88(23), 13918–13922. http://doi.org/10.1128/JVI.01615-14https://hdl.handle.net/1805/8966Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24−/− cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.en-USPublisher PolicyAnimalsCell LineCholesterolDesmosterolHerpesvirus 1, HumanVirus InternalizationArticle