Pandya, Pankita H.Fisher, Amanda J.Mickler, Elizabeth A.Temm, Constance J.Lipking, Kelsey P.Gracon, AdamRothhaar, KatiaSandusky, George E.Murray, MaryPollok, KarenRenbarger, JamieBlum, Janice S.Lahm, TimWilkes, David S.2018-08-022018-08-022016-12Pandya, P. H., Fisher, A. J., Mickler, E. A., Temm, C. J., Lipking, K. P., Gracon, A., … Wilkes, D. S. (2016). Hypoxia-Inducible Factor-1α Regulates CD55 in Airway Epithelium. American Journal of Respiratory Cell and Molecular Biology, 55(6), 889–898. https://doi.org/10.1165/rcmb.2015-0237OC1044-1549https://hdl.handle.net/1805/16945Airway epithelial CD55 down-regulation occurs in several hypoxia-associated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxia-inducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on small-airway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxia-recovered CD55 expression in small-airway epithelial cells. HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium.en-USPublisher Policycomplement regulatory proteinshypoxialungArticle