Reiter, Jill L.Drendel, Holli M.Chakraborty, SujataSchellinger, Megan M.Lee, Men-JeanMor, Gil2017-03-312017-03-312017-04Reiter, J. L., H. M. Drendel, S. Chakraborty, M. M. Schellinger, M.-J. Lee and G. Mor (2017). Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE. Placenta. 52, 17-20. http://dx.doi.org/10.1016/j.placenta.2017.02.010https://hdl.handle.net/1805/12163Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells.enIUPUI Open Access Policytrophoblast cell linescellular immortalizationtelomeraseArticle