Sebastiani, GuidoGrieco, Giuseppina EmanuelaBruttini, MarcoAuddino, StefanoMori, AlessiaToniolli, MattiaFignani, DanielaLicata, GiadaAiello, ElenaNigi, LauraFormichi, CaterinaFernandez-Tajes, JuanPugliese, AlbertoEvans-Molina, CarmellaOverbergh, LutTree, TimothyPeakman, MarkMathieu, ChantalDotta, FrancescoINNODIA investigators2024-09-232024-09-232024Sebastiani G, Grieco GE, Bruttini M, et al. A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes. Cell Rep Med. 2024;5(6):101591. doi:10.1016/j.xcrm.2024.101591https://hdl.handle.net/1805/43489Circulating microRNAs (miRNAs) are linked to the onset and progression of type 1 diabetes mellitus (T1DM), thus representing potential disease biomarkers. In this study, we employed a multiplatform sequencing approach to analyze circulating miRNAs in an extended cohort of prospectively evaluated recent-onset T1DM individuals from the INNODIA consortium. Our findings reveal that a set of miRNAs located within T1DM susceptibility chromosomal locus 14q32 distinguishes two subgroups of individuals. To validate our results, we conducted additional analyses on a second cohort of T1DM individuals, confirming the identification of these subgroups, which we have named cluster A and cluster B. Remarkably, cluster B T1DM individuals, who exhibit increased expression of a set of 14q32 miRNAs, show better glycemic control and display a different blood immunomics profile. Our findings suggest that this set of circulating miRNAs can identify two different T1DM subgroups with distinct blood immunomics at baseline and clinical outcomes during follow-up.en-USAttribution-NonCommercial-NoDerivatives 4.0 InternationalBiomarkersChromosome 14q32EndotypeHeterogeneityImmunomicsMicroRNAsPlasmaSmall RNA-seqType 1 diabetesArticle