Rizk, Shahir S.Mukherjee, SomnathKoide, AkikoKoide, ShoheiKossiakoff, Anthony A.2017-08-242017-08-242017-04-03Rizk, S. S., Mukherjee, S., Koide, A., Koide, S., & Kossiakoff, A. A. (2017). Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody. Scientific Reports, 7, 556. http://doi.org/10.1038/s41598-017-00728-1https://hdl.handle.net/1805/13909We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). We show that a conformation-specific Fab can reactivate an IDH1 mutant associated with brain tumors. The results show that this strategy is a first step towards developing "activator drugs" for a large number of genetic disorders where mutations have disrupted protein function.en-USAttribution-NonCommercial-NoDerivs 3.0 United StatesAntibody fragments (Fabs)Enzyme isocitrate dehydrogenase 1 (IDH1)IDH1 mutant associated with brain tumorsProtein functionMutationsArticle