Mumaw, Christen L.Surace, MichaelLevesque, ShannonKodavanti, Urmila P.Kodavanti, Prasada Rao S.Royland, Joyce E.Block, Michelle L.2019-12-202019-12-202017-03Mumaw, C. L., Surace, M., Levesque, S., Kodavanti, U. P., Kodavanti, P., Royland, J. E., & Block, M. L. (2017). Atypical microglial response to biodiesel exhaust in healthy and hypertensive rats. Neurotoxicology, 59, 155–163. doi:10.1016/j.neuro.2016.10.012https://hdl.handle.net/1805/21529Accumulating evidence suggests a deleterious role for urban air pollution in central nervous system (CNS) diseases and neurodevelopmental disorders. Microglia, the resident innate immune cells and sentinels in the brain, are a common source of neuroinflammation and are implicated in how air pollution may exert CNS effects. While renewable energy, such as soy-based biofuel, is of increasing public interest, there is little information on how soy biofuel may affect the brain. To address this, male spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats were exposed to 100% Soy Biodiesel Exhaust (100SBDE; 0, 50, 150 and 500 μg/m3) by inhalation for 4 h/day for 4 weeks (5 days/week). IBA-1 staining of microglia in the substantia nigra revealed significant changes in morphology with 100SBDE exposure in rats from both genotypes, where the SHR were less sensitive. Further analysis failed to show consistent changes in pro-inflammatory cytokine expression, nitrated protein, and arginase1 expression in brain tissue from either rat strain exposed to 100SBDE. CX3CR1 and fractalkine mRNA expression were lower in the striatum of all 100SBDE exposed rats, but greater SBDE exposure was required for loss of fractalkine expression in the SHR. Together, these data support that month-long 100SBDE exposure impacts the basal ganglia with changes in microglia morphology, an impaired fractalkine axis, and an atypical activation response without traditional markers of M1 or M2 activation, where the SHR may be less sensitive to these effects.en-USPublisher PolicyBiodieselAir PollutionBrainMicrogliaAtypical ActivationArticle